| Author: | Yang, Suhan |
| Title: | DNA absorption and desorption with functionalized nanoporous anodic aluminum oxide membrane |
| Degree: | M.Sc. |
| Year: | 2013 |
| Subject: | Mobile genetic elements. DNA -- drug effects. Hong Kong Polytechnic University -- Dissertations |
| Department: | Interdisciplinary Division of Biomedical Engineering |
| Pages: | ix, 49 leaves : ill. (some col.) ; 30 cm. |
| Language: | English |
| Abstract: | DNA extraction and purification from vivo sample is demanded in various research fields for genetic analysis process. Characterized by its chemical stability, potentials in diversified surface modification options and simplified protocols, solid-phase based method for DNA molecules isolation has widely discussed. In this project, a novel biomembrane: nanoporous anodic aluminum oxide (AAO) membrane was employed as DNA extraction substrate after APTES modification to investigate its optimal interaction conditions and to exam the different Lambda DNA binding capacities among three pore sizes. The best absorption and desorption conditions were pH 4.5 and pH 10.5, under these reaction conditions, Lambda DNA capturing ability increased with the amino group intensity. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b25583797.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 7.87 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/6872