| Author: | Ma, Chi Wah Jerry |
| Title: | Evaluation of direct detection of mycobacterium tuberculosis complex in extra-pulmonary clinical specimens with Cepheid Genexpert platform |
| Advisors: | Siu, Gilman (HTI) |
| Degree: | M.Sc. |
| Year: | 2015 |
| Subject: | Tuberculosis -- Diagnosis Mycobacterium tuberculosis -- Diagnosis Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | vii, 45 leaves ; 30 cm |
| Language: | English |
| Abstract: | Extrapulmonary tuberculosis (EPTB) is considered a major clinical problem and has been noted to have significantly increased worldwide, especially in industrialized countries, which accounts for approximately 20% of Tuberculosis (TB) cases. Xpert MTB/RIF has been proven to be an excellent point-of-care platform to diagnose pulmonary TB in highly endemic countries with a fully automatic molecular system. In this study, we aimed to investigate the ability of the Xpert system to detect extrapulmonary tuberculosis in Hong Kong. We included 231 extrapulmonary clinical samples which correspond to 188 patients. By comparing with the gold standard culture method as well as the clinical diagnosis of TB records, an overall sensitivity of 81.8% and specificity of 100% was obtained. For the biopsy specimens, the sensitivity is around 80% while the non-biopsy specimens including the body fluid, pus & urine was 100%. The performance of Xpert is better in the AFB microscopy positive than in the negative sample, with a sensitivity of 100% in the positive compared to 75% in the negative samples. Even though the conventional culture method is still considered the main tool in the diagnosis of extrapulmonary TB, the Xpert system provides an alternative method to screen for EPTB in a more efficient way with higher sensitivity and faster turnaround time compared with smear microscopy. Hence, including traditional culture methods as well as the Xpert as a combo test for the screening of EPTB is recommended. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b28001576.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 712.29 kB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/7906