| Author: | Tan, Xiaoying |
| Title: | Graphene quantum dots-aptamer and graphene oxide based fluorescence resonance energy transfer biosensor for the detection of EpCAM protein and breast cancer cell (MCF-7) |
| Advisors: | Yang, Mo (BME) |
| Degree: | M.Sc. |
| Year: | 2015 |
| Subject: | Biosensors. Fluorescent probes. Fluorescence. Hong Kong Polytechnic University -- Dissertations |
| Department: | Interdisciplinary Division of Biomedical Engineering |
| Pages: | x, 59 pages : color illustrations |
| Language: | English |
| Abstract: | Fluorescence resonance energy transfer (FRET) is a nano-scale technique that has already been developed since the early years. Nowadays, FRET phenomenon has been used in biosensing, imaging and diseases treatment mutually. This study introduces a novel material to construct FRET biosensing platform for protein and cell detection. Comparing to traditional organic donors, graphene quantum dots bring various benefits for biosensor such as stable optical properties and longer life time. And with a wide absorption spectrum and excellent quenching ability, graphene oxide is as a perfect quencher in FRET biosensor. In this dissertation, graphene quantum dots functionalized by aptamer and graphene oxide based FRET biosensor platform was developed for sensitive and specific detection of EpCAM protein and MCF-7 breast cancer cells. The GQDs-aptamer acts as the donor is quenched by GO acceptor. As the interaction of GQDs-aptamer complex with GO is broken in favour for a stronger and more specific binding with the target protein, the fluorescent signal was restored and the existence of target was detected sensitively and selectively. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b28391901.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 1.74 MB | Adobe PDF | View/Open |
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